PhD Oral Qualifying Examination : Metabolic processes of Methanococcus maripaludis S2

Speaker Vo Chi Hung (Supervisor(s): Prof Karimi IA & Prof Markus Kraft (University of Cambridge)

Host Department of Chemical and Biomolecular Engineering

Date/Time 13 Jul - 13 Jul, 10.00am

Venue E5-02-32 , Faculty of Engineering, National University of Singapore


Over the past decades, the global anthropogenic CO2 emission has steadily increased, garnering the attention to CO2 capture, storage and utilization. In the area of microbial fixation of CO2, microbes which utilise the Calvin cycle such as algae and cyanobacteria have been the focus of most studies. Not much research has been done on the archaeal methanogens, which can fix CO2 through the Wood–Ljungdahl pathway. In particular, M. maripaludis is the microbe of interest. This archaeon is methanogenic and mesophilic, with a doubling time of two hours. In addition, its genome has been fully sequenced. Genome-scale metabolic models for M. maripaludis have been built to facilitate understanding, optimization and design. However, the current models are limited to steady-state and validation from gas phase concentrations. In my study, I investigate the growth of M. maripaludis in nutrient-rich and nutrient-deficient environments. Both gas phase and liquid phase are analysed to develop a genome-scale metabolic model for this archaeon.