Photoactivatable AIE Polymer for Light Controlled Gene Delivery: Concurrent Endo-/Lysosomal Escaping and DNA Unpacking

Endo-/lysosomal escaping of gene vectors and the subsequent unpacking of nucleic acid in cytosol are two major challenges for efficient gene delivery. Herein, we report a polymeric gene delivery vector, which consists of a photosensitizer (PS) with aggregation-induced emission (AIE) characteristics and oligoethylenimine (OEI) conjugated via a reactive oxygen species (ROS) cleavable aminoacrylate (AA) linker. The polymer could self-assemble into bright red fluorescent nanoparticles (NPs) in aqueous media, which can efficiently bind to DNA through electrostatic interaction for gene delivery. Upon visible light irradiation, the generated ROS can break the endo-/lysosomal membrane and the polymer, resulting in light-controlled endo-/lysosomal escaping and unpacking of DNA for efficient gene delivery. The smart polymer represents the first successful gene vector to simultaneously address both challenges with single light excitation.

 

Photoactivatable AIE Polymer for Light Controlled Gene Delivery: Concurrent Endo-/Lysosomal Escaping and DNA Unpacking

 A novel photo-active polymeric gene delivery vector that can concurrently escape from the endo-/lysosome and undergo unpacking of nucleic acid in cytosol upon single light irradiation has been developed for light-controlled gene delivery

 

This work has been published in Angewandte Chemie International Edition on June 19, 2015 (doi: 10.1002/anie.201503640)

Corresponding author(s): Bin Liu ( This email address is being protected from spambots. You need JavaScript enabled to view it. )
Corresponding author(s) webpage: http://cheed.nus.edu.sg/stf/cheliub/