Organic NPs with Tunable Size for Evaluation of Blood-Brain Barrier Damage’
Detection of blood-brain barrier (BBB) damage is important for the brain disease diagnosis. The most widely used probe, Evans blue, suffers from low specificity and high toxicity. In this work, organic nanoparticles (NPs) with aggregation induced emission characteristics and controlled sizes (60 nm, 30 nm and 10 nm) were synthesized by modified nanoprecipitation methods. In a rat model of photothrombotic ischemia (PTI), the extent of NP leakage in various brain locations in response to BBB damage was evaluated by fluorescence imaging. The 30 nm NPs were found to be the most sensitive and selective probes for the BBB damage evaluation, while the 60 nm NPs can barely pass through BBB and the 10 nm NPs show relatively poor selectivity, which proves the size-dependent permeability of BBB after disruption. Attributing to their high brightness and tunable sizes, these NPs could also function as excellent selective probes for the assessment of BBB damage induced by other neurological diseases.
This work has been published in Adv. Mater. On August 11, 2016 (doi:10.1002/adma.201601191)
Corresponding author(s): Bin, Liu (
Corresponding authors(s) Webpage: http://cheed.nus.edu.sg/stf/cheliub/